Advanced Biochemistry I
BI3174/BI6184
(Fall Semester)

The course aims at introducing advanced concepts in biochemistry and provides a framework to understand basic biomolecular function. Its special emphasis is on studying enzymes as biological catalysts and understanding the chemistry of enzymatic transformations. It also deals with routine and advanced methodologies used in generating and analyzing biochemical data. This course is meant to help understand biology from the point of view of molecules and physicochemical principles. I co-teach this course with Sudha Rajamani

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Topics covered by me include: 

Module 1: Enzyme biochemistry: enzyme catalyzed reactions, steady state enzyme kinetics, enzyme inhibition, multisite and allosteric enzymes, pH rate profiles, viscosity effects, isotopic effects in enzyme catalysis, Cleland notations, pre-steady state enzyme kinetics

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Module 2: Enzyme catalyzed reactions: hydrolysis of peptides bonds, phosphate transfer and hydrolysis, the amidohydrolase family, the enolase family, the amidotransferases & molecular tunnels, NAD catalyzed reactions, and the radical SAM superfamily

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Recommended books:

1. Enzyme kinetics and mechanism, by Paul Cook & W. W. Cleland

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2. Enzyme reaction mechanisms, by Perry Frey & Adrian D. Hegeman.